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glial cell nuclei  (Developmental Studies Hybridoma Bank)


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    Developmental Studies Hybridoma Bank glial cell nuclei
    Glial Cell Nuclei, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/glial+nuclei/anti-Nuclei/pm35990742-55-13-19
    Average 94 stars, based on 24 article reviews
    glial cell nuclei - by Bioz Stars, 2026-09
    94/100 stars

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    Article Title: Alignment between glioblastoma internal clock and environmental cues ameliorates survival in Drosophila.
    Article Snippet: Adult brains were dissected and fixed with 4% formaldehyde in phosphate-buffered saline for 20 min whereas adult NMJ were fixed for 10 min; in both cases, samples were washed 3 × 15min with 10 COMMUNICATIONS BIOLOGY | (2022) 5:644 | https://doi.org/10.1038/s42003-022-03600-9 | www.nature.com/commsbio PBS+ 0.4% triton, blocked for 1 h with PBS+ 0.4% triton+BSA 5%, incubated overnight with primary antibodies, washed 3 × 15min, incubated with secondary antibodies for 2 h and mounted in Vectashield mounting medium, with DAPI in the case of the brains.0.4% triton, blocked for 1 h with PBS+ 0.4% triton+BSA 5%, incubated overnight with primary antibodies, washed 3 × 15min, incubated with secondary antibodies for 2 h and mounted in Vectashield mounting medium, with DAPI in the case of the brains. ... The primary antibodies used were mouse anti-repo (1:200, DSHB) to recognize glial nuclei, mouse anti-bruchpilot (NC82- 1:50, DSHB) to recognize the presynaptic protein Bruchpilot, rabbit anti-HRP (1/400, Cell Signalling) to recognize membranes, mouse anti-pdf (DHSB, 1:2000), rabbit anti-GFP (1:500, DSHB) and mouse anti-Elav (1:100, DSHB) to recognize neuron nuclei.. The secondary antibodies used were Alexa-488 or 647 (1:500, Life Technologies).The secondary antibodies used were Alexa-488 or 647 (1:500, Life Technologies).

    Article Title: Alignment between glioblastoma internal clock and environmental cues ameliorates survival in Drosophila
    Article Snippet: Adult brains were dissected and fixed with 4% formaldehyde in phosphate-buffered saline for 20 min whereas adult NMJ were fixed for 10 min; in both cases, samples were washed 3 × 15 min with PBS + 0.4% triton, blocked for 1 h with PBS + 0.4% triton+BSA 5%, incubated overnight with primary antibodies, washed 3 × 15 min, incubated with secondary antibodies for 2 h and mounted in Vectashield mounting medium, with DAPI in the case of the brains.blocked for 1 h with PBS + 0.4% triton+BSA 5%, incubated overnight with primary antibodies, washed 3 × 15 min, incubated with secondary antibodies for 2 h and mounted in Vectashield mounting medium, with DAPI in the case of the brains. ... The primary antibodies used were mouse anti-repo (1:200, DSHB) to recognize glial nuclei, mouse anti-bruchpilot (NC82- 1:50, DSHB) to recognize the presynaptic protein Bruchpilot, rabbit anti-HRP (1/400, Cell Signalling) to recognize membranes, mouse anti-pdf (DHSB, 1:2000), rabbit anti-GFP (1:500, DSHB) and mouse anti-Elav (1:100, DSHB) to recognize neuron nuclei.. The secondary antibodies used were Alexa-488 or 647 (1:500, Life Technologies).The secondary antibodies used were Alexa-488 or 647 (1:500, Life Technologies).

    Article Title: Circadian Gene cry Controls Tumorigenesis through Modulation of Myc Accumulation in Glioblastoma Cells
    Article Snippet: Adult brains were dissected and fixed with 4% formaldehyde in phosphate-buffered saline for 20 min, whereas adult NMJ were fixed for 10 min; in both cases, samples were washed 3 × 15 min with PBS + 0.4% triton, blocked for 1 h with PBS + 0.4% triton + BSA 5%, incubated overnight with primary antibodies, washed 3 × 15 min, incubated with secondary antibodies for 2 h and mounted in Vectashield mounting medium with DAPI in the case of the brains.triton + BSA 5%, incubated overnight with primary antibodies, washed 3 × 15 min, incubated with secondary antibodies for 2 h and mounted in Vectashield mounting medium with DAPI in the case of the brains. ... The primary antibodies used were anti-Repo mouse (1/200; DSHB, Iowa City, IA, USA) to recognize glial nuclei, anti-Bruchpilot-nc82-mouse (1/50; DSHB, Iowa City, IA, USA) to recognize the presynaptic protein Bruchpilot, anti-HRP rabbit (1/400; Cell Signaling, Danvers, MA, USA) to recognize neuronal membranes, anti-GFP rabbit (1:500; DSHB, Iowa City, IA, USA) and anti-Myc guinea pig (1/100; DSHB, Iowa City, IA, USA) to recognize the nuclear protein Myc.. The secondary antibodies used were anti-mouse, -rabbit or -guinea pig Alexa 488 or 647 (1/500; Life Technologies, Carlsbad, CA, USA).The secondary antibodies used were anti-mouse, -rabbit or -guinea pig Alexa 488 or 647 (1/500; Life Technologies, Carlsbad, CA, USA).

    Article Title: Circadian Gene <em>cry</em> Controls Glioblastoma Tumorigenesis through Modulation of <em>myc</em> Expression
    Article Snippet: Adult brains were dissected and fixed with 4% formaldehyde in phosphatebuffered saline for 20 min whereas adult NMJ were fixed 10 min; in both cases, samples were washed 3 × 15 min with PBS+0.4% triton, blocked for 1 h with PBS+0.4% triton+ BSA 5%, incubated overnight with primary antibodies, washed 3 × 15 min, incubated with secondary antibodies for 2 h, and mounted in Vectashield mounting medium, with DAPI in the case of the brains.PBS+0.4% triton, blocked for 1 h with PBS+0.4% triton+ BSA 5%, incubated overnight with primary antibodies, washed 3 × 15 min, incubated with secondary antibodies for 2 h, and mounted in Vectashield mounting medium, with DAPI in the case of the brains. ... The primary antibodies used were anti-repo mouse (1/200; DSHB) to recognize glial nuclei, anti-bruchpilot-NC82-mouse (1/50; DSHB) to recognize the presynaptic protein bruchpilot, anti-HRP rabbit (1/400; Cell Signaling) to recognize membranes, anti-GFP rabbit (1:500; DSHB), anti-Myc guinea pig (1/100; DSHB) to recognize the nuclear protein Myc,.. The secondary antibodies used were Alexa 488 or 647 (1/500; Life Technologies).The secondary antibodies used were Alexa 488 or 647 (1/500; Life Technologies).

    Article Title: Insulin signaling mediates neurodegeneration in glioma
    Article Snippet: Adult brains were dissected and fixed with 4% formaldehyde in phosphate-buffered saline for 20 min whereas adult NMJ were fixed 10 min; in both cases, samples were washed 3 × 15 min with PBS+0.4% triton, blocked for 1 h with PBS+0.4% triton+ BSA 5%, incubated overnight with primary antibodies, washed 3 × 15 min, incubated with secondary antibodies for 2 h, and mounted in Vectashield mounting medium, with DAPI in the case of the brains.min with PBS+0.4% triton, blocked for 1 h with PBS+0.4% triton+ BSA 5%, incubated overnight with primary antibodies, washed 3 × 15 min, incubated with secondary antibodies for 2 h, and mounted in Vectashield mounting medium, with DAPI in the case of the brains. ... The primary antibodies used were anti-repo mouse (1/200; DSHB) to recognize glial nuclei, anti-bruchpilot-NC82-mouse (1/50; DSHB) to recognize the presynaptic protein bruchpilot, anti-HRP rabbit (1/400; Cell Signalling) to recognize membranes, anti-GFP rabbit (1:500; DSHB) and anti-Elav (1:100; DSHB) to recognize neuron nuclei.. The secondary antibodies used were Alexa 488 or 647 (1/500; Life Technologies).The secondary antibodies used were Alexa 488 or 647 (1/500; Life Technologies).



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